By Martin Egli, Piet Herdewijn, Akira Matusda
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Extra resources for Current Protocols in Nucleic Acid Chemistry
3B). , YMC-ODS-AQ column; YMC) 1. Combine the following in a 12 × 75–mm glass test tube or conical vial with a small magnetic stir bar. 13 mmol) DIEA 200 µL DMSO. Synthesis of N2-Substituted Deoxyguanosine Nucleosides 2. 31 mmol) of 1,3-diaminopropane, cap securely, and heat while stirring in a 45°C oil bath or heating block. 12 Current Protocols in Nucleic Acid Chemistry Additional amine (well in excess of the 1 eq that is theoretically required) is used to ensure that only one of the amino groups in the diaminopropane reacts with the fluoronucleoside.
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And Lin, X. 1991. Palladium-mediated synthesis of C-5 pyrimidine nucleoside thioethers from disulfides and mercurinucleosides. J. Org. Chem. 56:5598-5602. C. T. 1995. Very high affinity DNA recognition by bicyclic and crosslinked oligonucleotides. J. Am. Chem. Soc. 117:10434-10442. L. 1988. Synthesis and hybridization of a series of biotinylated oligonucleotides. Nucl. Acids Res. 16:4077-4095. B. B. 1985. Fe(II). Proc. Natl. Acad. Sci. A. 82:968-972. J. J. 1987. Synthesis and application of derivatizable oligonucleotides.