Chemosensitivity Testing in Oncology by Uwe Reinhold, Wolfgang Tilgen

By Uwe Reinhold, Wolfgang Tilgen

The decade has noticeable a renaissance of the concept that of individualized chemotherapy in oncology, markedly influenced by means of the improvement of latest in vitro chemosensitivity assays. The medical software of drug reaction assays has been evaluated in scientific trials and the consequences recommend that assay-assisted treatment choice could enhance survival in addition to financial results. This quantity contains the court cases of the 1st Symposium of the foreign Society for Chemosensitivity trying out in Oncology, ISCO-1, held in Homburg/Saar, Germany, in September 2001. the themes comprise: new in vitro drug-testing equipment, tumor chemosensitivity assays, and the scientific relevance of assay-directed treatment.

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The addition of cytochalasin B to these cells, which not only results in disruption of the actin filaments but also inhibits glucose uptake, has profound and rapid effects on cellular morphology and metabolism (Fig. 2) (Ehret et al. 2001). , glycolysis. At the same time, there is an increased consumption of oxygen , which is indicative of a compensatory oxidative utilization of amino acids. The increased capacitance values of the impedance sensors in the cell Fig. 2. Effect of cytochalasin B on metabolic and morphological parameters of LS174T cells as detected by a multiparametric glass sensor chip.

For example, we sometimes find a cohort of patients whose tumour cells appear resistant in vitro and express a particular resistance marker which is susceptible to modulation with known inhibitors of that specific mechanism. However, more often than not this is not the case. The MTT assay, or indeed any cell survival assay, measures the total effect of all resistance mechanisms in play at any particular time, providing a snap-shot of each tumour. It is quite possible that when one resistance mechanism is inhibited the cell switches to the use of another pathway.

Cytotoxicity tests (trypan blue exclusion, LDH colorimetric test) 10. Fluorometric microculture cytotoxicity assays 11. Nuclear damage assay 12. Screening assay using nude mice and isotopic evaluation 13. In vitro chemosensitivity test based on immunohistochemical detection of certain enzymes [for instance, ODC (ornithine decarboxylase) as proliferation marker] in individual cells 14. In vitro chemosensitivity test using a three-dimensional culture matrix (collagen gel-embedded cells; sponge gel-supported histoculture drug-response assay, see also cytoprint assay) 15.

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