By John Swansbury
A set of key cytogenetic and FISH innovations utilized by smooth scientific laboratories within the genetic research of human malignancies. The book's sensible suggestion and techniques are appropriate to be used at each point of workmanship, together with absolutely tested laboratories, yet with a sympathetic bias in the direction of someone contemplating developing a brand new cytogenetics provider. right here the reader will locate not just hassle-free tutorials at the basics of human karyotypes and chromosome research, but additionally special discussions on how laboratories could optimally improve their repertoire of features to incorporate such more recent complementary suggestions as CGH, FISH, and M-FISH.
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Additional info for Cancer Cytogenetics. Methods and Protocols
Change the fixative shortly before spreading, then centrifuge again and add a few drops of fresh fixative to obtain a slightly cloudy suspension. Judging the correct dilution comes with experience: if the cells are not sufficiently dispersed, then the chromosomes will not spread or stain properly; if the dilution is too great then time will be wasted in screening nearly blank slides. 3. Generally just two slides are spread for each culture. Each slide should be labeled with the date, the patient’s ID, the type of culture, and a serial number.
There have been conflicting reports as to whether or not a trisomy 22 as the sole abnormality is likely to indicate the presence of a cryptic inv(16) (20,29). A del(16)(q22) is also a recurrent abnormality in myeloid malignancy; the interpretation of the significance of this abnormality requires more care, as in M4eo it is probably a variant of the inv(16) or t(16;16) and may indicate the same good prognosis; but in other conditions, such as MDS, it has been associated with a poor prognosis (30).
This is usually obtained as a powder. Cover a flask with aluminum foil, and insert a magnetic stirrer. 5 g of stain and 200 mL of methanol. Stir for 30 min. Filter through filter paper into a foil-coated bottle. Close the lid tightly, and store the bottle in a dark cupboard for at least a week before use. 8 buffer. 15. 5 is usually satisfactory. 16. Mounting medium: Gurr’s neutral mounting medium is routinely used in this laboratory; in our experience it does not leach stain if it 46 Swansbury not diluted with xylene.