Advances In Biochemical Engineering - Biotechnology - Modern

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Djordjevic NM, Fitzpatrick F, Houdiere F, Lerch G, Rozing G (2000) J Chromatogr A 887:245 152. Walhagen K, Unger KK, Hearn MTW (2000) J Chromatogr A 893:401 153. Huber CG, Choudhari G, Horváth C (1997) Anal Chem 69:4429 154. Breadmore MC, Macka M, Avdalovic N, Haddad PR (2000) Analyst 125:1235 155. Hilder EF, Macka M, Haddad PR (1999) Anal Commun 36:299 156. Klampfl CW, Haddad PR (2000) J Chromatogr A 884:277 157. Paull B, Nesterenko P, Nurdin M, Haddad PR (1998) Anal Commun 35:17 158. Xin B, Lee ML (1999) Electrophoresis 20:67 159.

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1 Mobile Phase Reversed-phase separations currently dominate in CEC. As a result, the vast majority of the mobile phases are mixtures of water and an organic solvent, typically acetonitrile or methanol. In addition to the modulation of the retention, the mobile phase in CEC also conducts electricity and must contain mobile ions. This is achieved by using aqueous mixtures of salts instead of pure water. The discussion in Sect. 2 of this chapter indicated that the electroosmotic flow is created by ionized functionalities.

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